A human iPSC model with LATS1/2 knockdown (MUSIi012-A-9) for investigating Hippo signaling in stem cell

Chanchao Lorthongpanich; Pimonwan Srisook; Nittaya Jiamvoraphong; Phatchanat Klaihmon; Siriluk Kumsui; Chuti Laowtammathron; Surapol Issaragrisil

doi:10.1016/j.scr.2026.104052

A human iPSC model with LATS1/2 knockdown (MUSIi012-A-9) for investigating Hippo signaling in stem cell

Chanchao Lorthongpanich
, Pimonwan Srisook
, Nittaya Jiamvoraphong
, Phatchanat Klaihmon
, Siriluk Kumsui
, Chuti Laowtammathron
, Surapol Issaragrisil

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Abstract

This study reports the generation and comprehensive characterization of the LATS1/2 knockdown (KD) induced pluripotent stem cell (iPSC) line, MUSIi012-A-9. Created via CRISPR/Cas9-mediated modification of the LATS2 gene in a parental LATS1-KD line, this resource serves as a crucial human model to investigate the roles of LATS1/2 kinases, key regulators of the Hippo signaling pathway. Comprehensive validation confirmed normal iPSC morphology, pluripotency marker expression (OCT3/4, NANOG, SOX2), genetic stability (46,XX karyotype), and robust multilineage differentiation potential. This well-characterized iPSC line is a vital tool for advancing research into Hippo pathway regulation, lineage specification, and cell fate determination in development and disease.

Original language	English
Article number	104052
Journal	Stem Cell Research
Volume	95
DOIs	https://doi.org/10.1016/j.scr.2026.104052
Publication status	Published - Sept 2026

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10.1016/j.scr.2026.104052

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title = "A human iPSC model with LATS1/2 knockdown (MUSIi012-A-9) for investigating Hippo signaling in stem cell",

abstract = "This study reports the generation and comprehensive characterization of the LATS1/2 knockdown (KD) induced pluripotent stem cell (iPSC) line, MUSIi012-A-9. Created via CRISPR/Cas9-mediated modification of the LATS2 gene in a parental LATS1-KD line, this resource serves as a crucial human model to investigate the roles of LATS1/2 kinases, key regulators of the Hippo signaling pathway. Comprehensive validation confirmed normal iPSC morphology, pluripotency marker expression (OCT3/4, NANOG, SOX2), genetic stability (46,XX karyotype), and robust multilineage differentiation potential. This well-characterized iPSC line is a vital tool for advancing research into Hippo pathway regulation, lineage specification, and cell fate determination in development and disease.",

author = "Chanchao Lorthongpanich and Pimonwan Srisook and Nittaya Jiamvoraphong and Phatchanat Klaihmon and Siriluk Kumsui and Chuti Laowtammathron and Surapol Issaragrisil",

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year = "2026",

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doi = "10.1016/j.scr.2026.104052",

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AU - Lorthongpanich, Chanchao

AU - Srisook, Pimonwan

AU - Jiamvoraphong, Nittaya

AU - Klaihmon, Phatchanat

AU - Kumsui, Siriluk

AU - Laowtammathron, Chuti

AU - Issaragrisil, Surapol

PY - 2026/9

Y1 - 2026/9

N2 - This study reports the generation and comprehensive characterization of the LATS1/2 knockdown (KD) induced pluripotent stem cell (iPSC) line, MUSIi012-A-9. Created via CRISPR/Cas9-mediated modification of the LATS2 gene in a parental LATS1-KD line, this resource serves as a crucial human model to investigate the roles of LATS1/2 kinases, key regulators of the Hippo signaling pathway. Comprehensive validation confirmed normal iPSC morphology, pluripotency marker expression (OCT3/4, NANOG, SOX2), genetic stability (46,XX karyotype), and robust multilineage differentiation potential. This well-characterized iPSC line is a vital tool for advancing research into Hippo pathway regulation, lineage specification, and cell fate determination in development and disease.

AB - This study reports the generation and comprehensive characterization of the LATS1/2 knockdown (KD) induced pluripotent stem cell (iPSC) line, MUSIi012-A-9. Created via CRISPR/Cas9-mediated modification of the LATS2 gene in a parental LATS1-KD line, this resource serves as a crucial human model to investigate the roles of LATS1/2 kinases, key regulators of the Hippo signaling pathway. Comprehensive validation confirmed normal iPSC morphology, pluripotency marker expression (OCT3/4, NANOG, SOX2), genetic stability (46,XX karyotype), and robust multilineage differentiation potential. This well-characterized iPSC line is a vital tool for advancing research into Hippo pathway regulation, lineage specification, and cell fate determination in development and disease.

UR - https://www.scopus.com/pages/publications/105043111697

U2 - 10.1016/j.scr.2026.104052

DO - 10.1016/j.scr.2026.104052

M3 - Article

AN - SCOPUS:105043111697

SN - 1873-5061

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JO - Stem Cell Research

JF - Stem Cell Research

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ER -

A human iPSC model with LATS1/2 knockdown (MUSIi012-A-9) for investigating Hippo signaling in stem cell

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