Abstract
Background: Cytokine-induced killer (CIK) cells are ex-vivo expanded T cells which present a phenotype of both T and Natural Killer cell properties. Objective: To compare the proliferation and functional properties of human CIK cells cultured in three cell culture plasticwares. Methods: The number and viability of CIK cells were monitored. The expression of surface markers (CD3 and CD56), TH1 cytokines (IFN-γ and TNF-α), and cytolytic granules (granzyme B and perforin) were determined by flow cytometry. Results: The number of CIK cells cultured in a static bag was highest compared to those in a petri dish and gas-permeable flask. However, CIK cells cultured in all plasticwares similarity expressed surface marker, TH1 cytokines, and cytolytic granules. Conclusion: Considering safety, efficacy, and cost, a static bag is the best plasticware for culturing CIK cells.
| Original language | English |
|---|---|
| Pages (from-to) | 401-406 |
| Number of pages | 6 |
| Journal | Asian Pacific Journal of Allergy and Immunology |
| Volume | 41 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Dec 2023 |
Keywords
- cell culture plasticware
- cytokine-induced killer cells
- gas-permeable flask
- immunotherapy
- static bag
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