Research output per year
Research output per year
Equipments Details
Description
Location: Microbiology Area, Research Center, Faculty of Medicine, Ramathibodi Hospital
Model: Axio Imager M2 (Fully Motorized Microscope)
White light source: LED
Illuminator microLED 2 1.7.1 423053-9072-000
Fluorescence light source: LED
Colibri 7, Type R[G/Y]B-UV 423052-9730-000
1. UV (385/30 nm - 370-400 nm) for excitation of DAPI, Hoechst 33342, Hoechst 33258, Alexa Fluor 350, Alexa Fluor 405, Indo-1, eBFP / BFP, eGFP (wt), True Blue and similar dyes
2. Blue (469/38 nm - 450-488 nm) for excitation of FM1-43, Cy2, eGFP, NBD, MitoTracker Green, Alexa Fluor 488, BCECF, Calcein, DiO SNAFL, YO-Pro-1, Nissl, LysoSensor Green, honeydew, FITC/ Fluorescein, Kaede (green /red), PerCP, YoYo-1, FuraRed and similar dyes
Model: Axio Imager M2 (Fully Motorized Microscope)
White light source: LED
Illuminator microLED 2 1.7.1 423053-9072-000
Fluorescence light source: LED
Colibri 7, Type R[G/Y]B-UV 423052-9730-000
1. UV (385/30 nm - 370-400 nm) for excitation of DAPI, Hoechst 33342, Hoechst 33258, Alexa Fluor 350, Alexa Fluor 405, Indo-1, eBFP / BFP, eGFP (wt), True Blue and similar dyes
2. Blue (469/38 nm - 450-488 nm) for excitation of FM1-43, Cy2, eGFP, NBD, MitoTracker Green, Alexa Fluor 488, BCECF, Calcein, DiO SNAFL, YO-Pro-1, Nissl, LysoSensor Green, honeydew, FITC/ Fluorescein, Kaede (green /red), PerCP, YoYo-1, FuraRed and similar dyes
3. Green (555/30 nm - 540-570 nm) for excitation of TRITC, 7-AAD, Cy3, tdTomato, Alexa Fluor 546, Alexa Fluor 555, DsRed, mOrange, TagRFP, SNARF, DyLight 549, Spectrum Orange and similar dyes
4. Yellow (590/27 nm - 576-603 nm) for excitation of MitoTracker RED FM/CMXRos, txRed, mCherry, mRFP1, Cy3.5, Rhodamine B, Alexa Fluor 568, Dylight 594, Alexa Fluor 594, Bodipy TR and similar dyes
5. Red (631/33 nm - 614-647 nm) for excitation of Alexa Fluor 633, Alexa Fluor 647, Cy5, DRAQ5, ToTo-3, ATTO-655, MitoTracker DeepRed, APC, ATTO-647N and similar dyes
Current filters:
1. Filterset 96 HE BFP
4. Yellow (590/27 nm - 576-603 nm) for excitation of MitoTracker RED FM/CMXRos, txRed, mCherry, mRFP1, Cy3.5, Rhodamine B, Alexa Fluor 568, Dylight 594, Alexa Fluor 594, Bodipy TR and similar dyes
5. Red (631/33 nm - 614-647 nm) for excitation of Alexa Fluor 633, Alexa Fluor 647, Cy5, DRAQ5, ToTo-3, ATTO-655, MitoTracker DeepRed, APC, ATTO-647N and similar dyes
Current filters:
1. Filterset 96 HE BFP
Item no: 489096-9100-000:
EX BP 390/40, BS FT 420, EM BP 450/40
https://www.micro-shop.zeiss.com/en/pl/fluorescenceAssistant/filtersets/489096-9100-000
2. Alexa flour 488 zone:Semrock
475/35 nm BrightLine® single-band bandpass filter
https://www.idex-hs.com/store/product-detail/ff01_475_35_25/fl-004539?search=true
495 nm edge BrightLine® single-edge standard epi-fluorescence dichroic beamsplitter
https://www.idex-hs.com/store/product-detail/ff495_di03_25x36/fl-007145?search=true
515/30 nm BrightLine® single-band bandpass filter
https://www.idex-hs.com/store/product-detail/ff01_515_30_25/fl-004382?search=true
3. Cy 3 Chroma 39004
EX AT540/25x, BS AT565DC, EM AT605/55m
4. 63 HE mRFP 489063-0000-000
EX BP 572/25 (HE) FT 590 (HE) BP629/62 (HE)
5. FL Filter Set 50 Cy5, EX BP640/30 S free
4. 63 HE mRFP 489063-0000-000
EX BP 572/25 (HE) FT 590 (HE) BP629/62 (HE)
5. FL Filter Set 50 Cy5, EX BP640/30 S free
Item no: 488050-9901-000
EX BP 640/30, BS FT 660, EM BP 690/50
https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/488050-9901-000
--
Previous filters:
1.Filter Set 02 DAPI 488002-9901-000
G 365 FT 395 LP 397
G 365 FT 395 LP 397
2.Filter set 44 FITC 000000-1114-459
EX BP 475/40, BS FT 500, EM BP 530/50
EX BP 475/40, BS FT 500, EM BP 530/50
3.90 HE DAPI/ GFP/ Cy3/ Cy5 489090-9110-000
EX BP 631/33
Beam splitter: QBS 405+493+575+653
Emission: QBP 425/30+514/30+592/25+709/100
--
Objective Lens:
1. N-Achroplan 5x/0.15 M27 (FWD=12.0mm) Item no.: 420930-9901-000
2. N-Achroplan 10x/0.25 M27 (FWD=6.5mm) Item no.: 420940-9901-000
3. N-Achroplan 20x/0.45 M27 (FWD=0.63mm) Item no.: 420950-9901-000
4. EC Plan-Neofluar 40x/0.75 M27 (FWD=0.71mm), incl. Cover glasses, high performance Item no.: 420360-9900-000
5. EC Plan-Neofluar 100x/1.30 Oil M27 (FWD=0.20mm), incl. Immersol 518 F Item no.: 420490-9900-000
Camera: 2 cameras
1. Color - ZEISS Axiocam 305 color
Sensor Model: Sony IMX 264 Exmor Pregius, CMOS
Sensor Pixel Count: 5 Megapixel: 2,464 (H) x 2,056 (V)
Pixel Size: 3.45 µm x 3.45 µm
Sensor Size: 8.5 mm x 7.1 mm; image diagonal 11.1 mm, equivalent to ⅔" sensor format
Spectral Range: Approx. 400 nm - 720 nm, coated Hoya C5000 IR Cut Filter; RGB Bayer color filter mask
2. Mono - ZEISS Axiocam 807 mono
Sensor TypeSony CMOS image sensor, Global Shutter architecture
Sensor SizeImage Diagonal 17.6 mm, equivalent to 1.1" Sensor Format
Image Field (14.5 mm x 9.9 mm)
Sensor Pixel Count3216 (H) x 2208 (V) = 7.1 Megapixel
HW Subsampling 2x1608 (H) x 1104 (V) = 1.8 Megapixel-high speed full view mode
Pixel Size 4.5 µm x 4.5 µm
Computer
Manual (Full) + Regulation - Require Mahidol VPN to access
https://www.rama.mahidol.ac.th/ramacloud/s/QuEavNgsKGJSrMQ
Booking and Logbook
https://minicore-rarc.blogspot.com/2018/05/upright-fluorescence-microscope.html
Operating manual + ZEN lite
https://drive.google.com/drive/folders/1JW6ljt3weToWRRFTTI4jhhm0iO-93fWt
1. N-Achroplan 5x/0.15 M27 (FWD=12.0mm) Item no.: 420930-9901-000
2. N-Achroplan 10x/0.25 M27 (FWD=6.5mm) Item no.: 420940-9901-000
3. N-Achroplan 20x/0.45 M27 (FWD=0.63mm) Item no.: 420950-9901-000
4. EC Plan-Neofluar 40x/0.75 M27 (FWD=0.71mm), incl. Cover glasses, high performance Item no.: 420360-9900-000
5. EC Plan-Neofluar 100x/1.30 Oil M27 (FWD=0.20mm), incl. Immersol 518 F Item no.: 420490-9900-000
Camera: 2 cameras
1. Color - ZEISS Axiocam 305 color
Sensor Model: Sony IMX 264 Exmor Pregius, CMOS
Sensor Pixel Count: 5 Megapixel: 2,464 (H) x 2,056 (V)
Pixel Size: 3.45 µm x 3.45 µm
Sensor Size: 8.5 mm x 7.1 mm; image diagonal 11.1 mm, equivalent to ⅔" sensor format
Spectral Range: Approx. 400 nm - 720 nm, coated Hoya C5000 IR Cut Filter; RGB Bayer color filter mask
2. Mono - ZEISS Axiocam 807 mono
Sensor TypeSony CMOS image sensor, Global Shutter architecture
Sensor SizeImage Diagonal 17.6 mm, equivalent to 1.1" Sensor Format
Image Field (14.5 mm x 9.9 mm)
Sensor Pixel Count3216 (H) x 2208 (V) = 7.1 Megapixel
HW Subsampling 2x1608 (H) x 1104 (V) = 1.8 Megapixel-high speed full view mode
Pixel Size 4.5 µm x 4.5 µm
Computer
OS NameMicrosoft Windows 11 Pro for Workstations
Version 10.0.26100 Build 26100
Other OS Description Not Available
OS ManufacturerMicrosoft Corporation
System Name DESKTOP-08TBNV5
System Manufacturer Dell Inc.
System ModelPrecision 5860 Tower
System Typex64-based PC
System SKU0A3C
ProcessorIntel(R) Xeon(R) w3-2423, 2112 Mhz, 6 Core(s), 12 Logical Processor(s)
BIOS Version/DateDell Inc. 2.2.9, 7/17/2024
SMBIOS Version3.4
Embedded Controller Version1.08
BIOS Mode UEFI
BaseBoard Manufacturer Dell Inc.
BaseBoard Product 0F4Y1M
BaseBoard Version A02
Locale United States
Hardware Abstraction Layer Version = "10.0.26100.1"
Installed Physical Memory (RAM) 32.0 GB
Total Physical Memory 31.3 GB
Available Physical Memory 23.8 GB
Total Virtual Memory 33.3 GB
Available Virtual Memory 25.2 GB
Graphic
Name NVIDIA T1000 8GB
PNP Device IDPCI\VEN_10DE&DEV_1FF0&SUBSYS_16121028&REV_A1\4&2FCCAACA&0&0008
Adapter TypeNVIDIA T1000 8GB, NVIDIA compatible
Adapter Description NVIDIA T1000 8GB
Adapter RAM (1,048,576) bytes
Driver Version 32.0.15.5612
INF File oem27.inf (Section014 section)
Color Planes Not Available
Color Table Entries4294967296
Resolution 3840 x 2160 x 59 hertz
Bits/Pixel 32
Disk drive: 2
1. ModelNVMe 3500 Micron 512G
Bytes/Sector512
Media LoadedYes
Media TypeFixed hard disk
Partitions4
PartitionDisk #1, Partition #0
Partition Size250.00 MB (262,144,000 bytes)
Partition Starting Offset1,048,576 bytes
PartitionDisk #1, Partition #1
Partition Size474.25 GB (509,222,060,032 bytes)
Partition Starting Offset397,410,304 bytes
PartitionDisk #1, Partition #2
Partition Size990.00 MB (1,038,090,240 bytes)
Partition Starting Offset509,619,470,336 bytes
PartitionDisk #1, Partition #3
Partition Size1.35 GB (1,451,229,184 bytes)
Partition Starting Offset510,658,609,152 bytes
2. Model ST2000NM012B-2TD130
Bytes/Sector512
Media LoadedYes
Media TypeFixed hard disk
Partitions1
PartitionDisk #0, Partition #0
Partition Size1.82 TB (2,000,263,577,600 bytes)
Partition Starting Offset135,266,304 bytes
Program: ZEN 3.10 (ZEN Pro)
https://www.rama.mahidol.ac.th/ramacloud/s/QuEavNgsKGJSrMQ
Booking and Logbook
https://minicore-rarc.blogspot.com/2018/05/upright-fluorescence-microscope.html
Operating manual + ZEN lite
https://drive.google.com/drive/folders/1JW6ljt3weToWRRFTTI4jhhm0iO-93fWt
Check crosstalk and Dye compatibility
https://www.fpbase.org/microscope/R3hqcsiTCe7D5rSueT8UsZ/ (new setting - use since 25Mar2026)
https://www.fpbase.org/microscope/DQNDPMbn4QfycdqkLhPizE/ (old setting)
Learning resource
https://www.youtube.com/playlist?list=PLVypea9Bb9IZY90X-WHV-wiSemHlqjCR4
Budget source:
National budget - Fiscal year 2024
BUDGET_PLAN - แผนงานยุทธศาสตร์เสริมสร้างให้คนมีสุขภาวะที่ดี
PROJECT - โครงการเพิ่มศักยภาพการให้บริการทางด้านสาธารณสุข
Amounts - ~3.5 millions baht
Code - https://github.com/tlerksuthirat/lab_management/blob/main/Budget_2567.ipynb
รหัสครุภัณฑ์
Axio Imager 2 (Fully Motorized): 13090002630
Dell Computer: 131000069017
Monitor dell: 131000069018
UPS APC: 131000069019
National budget - Fiscal year 2024
BUDGET_PLAN - แผนงานยุทธศาสตร์เสริมสร้างให้คนมีสุขภาวะที่ดี
PROJECT - โครงการเพิ่มศักยภาพการให้บริการทางด้านสาธารณสุข
Amounts - ~3.5 millions baht
Code - https://github.com/tlerksuthirat/lab_management/blob/main/Budget_2567.ipynb
รหัสครุภัณฑ์
Axio Imager 2 (Fully Motorized): 13090002630
Dell Computer: 131000069017
Monitor dell: 131000069018
UPS APC: 131000069019
Usage Report
https://github.com/tlerksuthirat/lab_management/blob/main/Motorized_Upright_Microscope_Usage.ipynb
Details
| Name | Motorized Upright Fluorescence Microscope |
|---|---|
| Acquisition date | 30/09/24 |
| Manufacturers | ZEISS |
Keywords
- RB Pathology
- QH301 Biology
- RC0254 Neoplasms. Tumors. Oncology (including Cancer)
- synthetic lethality
- QH426 Genetics
- DNA damage
- DNA repair
- DNA damage response
- RM Therapeutics. Pharmacology
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Fingerprint
Explore the research areas in which this equipment has been used. These labels are generated based on the related outputs. Together they form a unique fingerprint.
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Investigation of the Genotoxic Effects of Anti-Hepatitis B Virus Drugs and the Involvement of BRCA1 in DNA Damage Repair Pathways
Liekwannacharoen, S., Wikiniyadhanee, R., Lerksuthirat, T., Chitphuk, S. & Dejsuphong, D., 2026.Research output: Contribution to conference › Abstract › peer-review
Open Access -
Generation of an integration-free induced pluripotent stem cell line, MURAi006-A, from a hemoglobin E/β-thalassemia patient harboring the βE/β0 (Codon 17, A > T) compound heterozygous mutation
Innachai, P., Pornratananont, G., Satirapod, C., Anurathapan, U., Songdej, D., Tangprasittipap, A. & Hongeng, S., Jun 2025, In: Stem Cell Research. 85, 103702.Research output: Contribution to journal › Article › peer-review
Open Access
Activities
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iSEA Conference 2026
Lerksuthirat, T. (Member of programme committee)
22 Sept 2026 → 23 Sept 2026Activity: Participating in or organising an event › Organising a conference, workshop, ...
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The Versatility of a Bioimage Core Facility: A Multidisciplinary Approach to Biomedical Research
Lerksuthirat, T. (Speaker)
13 Nov 2025Activity: Talk or presentation › Invited talk
File -
iSEA Conference 2025
Lerksuthirat, T. (Member of programme committee)
12 Nov 2025 → 13 Nov 2025Activity: Participating in or organising an event › Organising a conference, workshop, ...
File
Press/Media
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Imaging Southeast Asia (iSEA): A New Regional Network Empowering Bioimaging Scientists
22/12/25
1 Media contribution
Press/Media